This page is provided for convinience, but it is reccomended that you verify manually with your actual trace files to determine whether a mutation is not a Sanger sequencing artefact. My reccomendation is that you open the ABI files and check what each mutation listed below looks like.
then twice (for forward and reverse): seq, option for no change, add (primer) or remove (barcode)
results for alignement
Does this align it? The alignment is pairwise and need not be sophisticated. 2 consecutive mismatchs. insertion/deletion. Trim, find 5 conserved.
AB file to XML. what if one could show the base in question. it depends on the XML
Wild type sequence